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Registro Completo |
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Biblioteca(s): |
Embrapa Algodão; Embrapa Café. |
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Data corrente: |
13/03/2014 |
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Data da última atualização: |
13/03/2014 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DIOLA, V.; BRITO, G. G. de; CAIXETA, E. T.; PEREIRA, L. F. P.; LOUREIRO, M. E. |
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Afiliação: |
VALDIR DIOLA, UFV - VIÇOSA; GIOVANI GREIGH DE BRITO, CNPA; EVELINE TEIXEIRA CAIXETA, SAPC; LUIZ FILIPE PROTASIO PEREIRA, SAPC; MARCELO E. LOUREIRO, UFV - VIÇOSA. |
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Título: |
A new set of differentially expressed signaling genes is early expressed in coffee leaf rust race II incompatible interaction. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Functional & Integrative Genomics, v. 13, n. 3, p. 379-389, 2013. |
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Idioma: |
Inglês
Português |
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Conteúdo: |
New races of coffee rust are overcoming resistance genes available in germplasm and cultivated cultivars and bringing recently some coffee-producing countries in severe economic challenge. The objective of this study was to identify the genes that are linked to host resistance to the major coffee rust race II. In our study, we have identified and studied a segregating population that has a single monogenic resistant gene to coffee rust. Coffee leaves of parents, resistant, and susceptible genotypes of the F2 generation plants were inoculated with pathogen spores. A differential analysis was performed by combined cDNA-AFLP and bulk segregant analysis (BSA) in pooled samples collected 48 and 72 h postinoculation, increasing the selectiveness for differential gene expression. Of 108 differential expressed genes, between 33,000 gene fragments analyzed, 108 differential expressed genes were identified in resistant plants. About 20 and 22 % of these resistant-correlated genes are related to signaling and defense genes, respectively. Between signaling genes, the major subclass corresponds to receptor and resistant homolog genes, like nucleotide-binding site leucine-rich repeat (NBSLRR), Pto-like, RLKs, Bger, and RGH1A, all not previously described in coffee rust responses. The second major subclass included kinases, where two mitogen-activated kinases (MAPK) are identified. Further gene expression analysis was performed for 21 selected genes by real-time PCR gene expression analysis at 0, 12, 24, 48, and 72 h postinoculation. The expression of genes involved in signaling and defense was higher at 24 and 72 h after inoculation, respectively. The NBS-LRR was the more differentially expressed gene between the signaling genes (four times more expressed in the resistant genotype), and thraumatin (PR5) was the more expressed between all genes (six times more expressed). Multivariate analysis reinforces the significance of the temporal separation of identified signaling and defense genes: early expression of signaling genes support the hypothesis that higher expression of the signaling components up regulates the defense genes identified. Additionally the increased gene expression of these two gene sets is associated with a single monogenic resistance trait to to leaf coffee rust in the nteraction characterized here. MenosNew races of coffee rust are overcoming resistance genes available in germplasm and cultivated cultivars and bringing recently some coffee-producing countries in severe economic challenge. The objective of this study was to identify the genes that are linked to host resistance to the major coffee rust race II. In our study, we have identified and studied a segregating population that has a single monogenic resistant gene to coffee rust. Coffee leaves of parents, resistant, and susceptible genotypes of the F2 generation plants were inoculated with pathogen spores. A differential analysis was performed by combined cDNA-AFLP and bulk segregant analysis (BSA) in pooled samples collected 48 and 72 h postinoculation, increasing the selectiveness for differential gene expression. Of 108 differential expressed genes, between 33,000 gene fragments analyzed, 108 differential expressed genes were identified in resistant plants. About 20 and 22 % of these resistant-correlated genes are related to signaling and defense genes, respectively. Between signaling genes, the major subclass corresponds to receptor and resistant homolog genes, like nucleotide-binding site leucine-rich repeat (NBSLRR), Pto-like, RLKs, Bger, and RGH1A, all not previously described in coffee rust responses. The second major subclass included kinases, where two mitogen-activated kinases (MAPK) are identified. Further gene expression analysis was performed for 21 selected genes by real-time PCR gene expression analysi... Mostrar Tudo |
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Palavras-Chave: |
Defense response; Host-specific resistance; R-genes; Transcript-derived fragments. |
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Thesagro: |
Hemileia Vastatrix. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/99182/1/functional.pdf
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Marc: |
LEADER 03085naa a2200229 a 4500
001 1982262
005 2014-03-13
008 2013 bl uuuu u00u1 u #d
100 1 $aDIOLA, V.
245 $aA new set of differentially expressed signaling genes is early expressed in coffee leaf rust race II incompatible interaction.$h[electronic resource]
260 $c2013
520 $aNew races of coffee rust are overcoming resistance genes available in germplasm and cultivated cultivars and bringing recently some coffee-producing countries in severe economic challenge. The objective of this study was to identify the genes that are linked to host resistance to the major coffee rust race II. In our study, we have identified and studied a segregating population that has a single monogenic resistant gene to coffee rust. Coffee leaves of parents, resistant, and susceptible genotypes of the F2 generation plants were inoculated with pathogen spores. A differential analysis was performed by combined cDNA-AFLP and bulk segregant analysis (BSA) in pooled samples collected 48 and 72 h postinoculation, increasing the selectiveness for differential gene expression. Of 108 differential expressed genes, between 33,000 gene fragments analyzed, 108 differential expressed genes were identified in resistant plants. About 20 and 22 % of these resistant-correlated genes are related to signaling and defense genes, respectively. Between signaling genes, the major subclass corresponds to receptor and resistant homolog genes, like nucleotide-binding site leucine-rich repeat (NBSLRR), Pto-like, RLKs, Bger, and RGH1A, all not previously described in coffee rust responses. The second major subclass included kinases, where two mitogen-activated kinases (MAPK) are identified. Further gene expression analysis was performed for 21 selected genes by real-time PCR gene expression analysis at 0, 12, 24, 48, and 72 h postinoculation. The expression of genes involved in signaling and defense was higher at 24 and 72 h after inoculation, respectively. The NBS-LRR was the more differentially expressed gene between the signaling genes (four times more expressed in the resistant genotype), and thraumatin (PR5) was the more expressed between all genes (six times more expressed). Multivariate analysis reinforces the significance of the temporal separation of identified signaling and defense genes: early expression of signaling genes support the hypothesis that higher expression of the signaling components up regulates the defense genes identified. Additionally the increased gene expression of these two gene sets is associated with a single monogenic resistance trait to to leaf coffee rust in the nteraction characterized here.
650 $aHemileia Vastatrix
653 $aDefense response
653 $aHost-specific resistance
653 $aR-genes
653 $aTranscript-derived fragments
700 1 $aBRITO, G. G. de
700 1 $aCAIXETA, E. T.
700 1 $aPEREIRA, L. F. P.
700 1 $aLOUREIRO, M. E.
773 $tFunctional & Integrative Genomics$gv. 13, n. 3, p. 379-389, 2013.
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Registro original: |
Embrapa Algodão (CNPA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
04/10/2018 |
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Data da última atualização: |
16/07/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
CANOVA, A. J. B.; SILVA, J. L. da; SOUZA, R. T. de; CONCEIÇÃO, M. A. F. |
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Afiliação: |
Anderson José Bocchi Canova, Estudante de graduação do curso de Agronomia da Universidade Brasil, Fernandópolis, SP, Estagiário Embrapa Uva e Vinho/EVT, Jales, SP. E-mail: aaanderson70@hotmail.com; Jéssica Luiza da Silva, Estudante de graduação do curso de Tecnologia em Agronegócio da FATEC Jales, Bolsista PIBIC/CNPq, Embrapa Uva e Vinho/EVT, Jales, SP; REGINALDO TEODORO DE SOUZA, CNPUV; MARCO ANTONIO FONSECA CONCEICAO, CNPUV. |
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Título: |
Brotação e enraizamento de diferentes cultivares de porta-enxertos de videiras na região noroeste do Estado de São Paulo. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 16.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 12., 2018, Bento Gonçalves. Anais... Bento Gonçalves, RS: Embrapa Uva e Vinho, 26 a 27 de setembro de 2018. p. 36. |
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Idioma: |
Português |
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Conteúdo: |
Para a produção de mudas de porta-enxertos de videiras deve-se considerar a capacidade de brotação e enraizamento dos mesmos. Sendo assim, o objetivo deste trabalho foi verificar a viabilidade de produção de mudas de porta-enxertos das cultivares IAC-572, IAC-766 e Freedom, nas condições climáticas da região noroeste do Estado de São Paulo. |
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Palavras-Chave: |
Anais; CNPUV; IC; Iniciação cientifica; Videira. |
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Thesagro: |
Brotacao; Porta Enxerto; Uva. |
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Categoria do assunto: |
A Sistemas de Cultivo |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/183946/1/Iniciacao-Cientifica-2018-Capa.cdr-Documentos-108-p36.pdf
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Marc: |
LEADER 01230nam a2200241 a 4500
001 2096928
005 2019-07-16
008 2018 bl uuuu u00u1 u #d
100 1 $aCANOVA, A. J. B.
245 $aBrotação e enraizamento de diferentes cultivares de porta-enxertos de videiras na região noroeste do Estado de São Paulo.$h[electronic resource]
260 $aIn: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 16.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 12., 2018, Bento Gonçalves. Anais... Bento Gonçalves, RS: Embrapa Uva e Vinho, 26 a 27 de setembro de 2018. p. 36.$c2018
520 $aPara a produção de mudas de porta-enxertos de videiras deve-se considerar a capacidade de brotação e enraizamento dos mesmos. Sendo assim, o objetivo deste trabalho foi verificar a viabilidade de produção de mudas de porta-enxertos das cultivares IAC-572, IAC-766 e Freedom, nas condições climáticas da região noroeste do Estado de São Paulo.
650 $aBrotacao
650 $aPorta Enxerto
650 $aUva
653 $aAnais
653 $aCNPUV
653 $aIC
653 $aIniciação cientifica
653 $aVideira
700 1 $aSILVA, J. L. da
700 1 $aSOUZA, R. T. de
700 1 $aCONCEIÇÃO, M. A. F.
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Embrapa Uva e Vinho (CNPUV) |
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